Rehabilitation DentistryMolecular and Regenerative Prosthodontics

Our major research focus is the development of next-generation biotechnology to 1) regenerate missing alveolar bone and teeth for functional and esthetic rehabilitation using cells and biomimetic materials and 2) introduce tailor-made diagnostics for prosthetic and implant treatments to prevent further tooth loss.


Topics of Research

  • iPS cell-based oral tissue engineering
  • Development of gingiva-derived iPS cells for safe therapeutic application
  • Biomimetic materials for bone tissue engineering
  • Chemical biology for bone regenerative medicine
  • Osteo-immunology in alveolar bone resorption
  • Development of genome-based diagnostics for prosthetic/implant treatments
  • Basic and clinical research on CAD/CAM-generated restorations
  • Periodontal treatments using free radicals and active oxygen generated by light energy

Recent Publications

  1. Niibe K, Ohori-Morita Y, Zhang M, Mabuchi Y, Matsuzaki Y, Egusa H: A shaking-culture method for generating bone marrow derived mesenchymal stromal/stem cell-spheroids with enhanced multipotency in vitro. Front Bioeng Biotechnol, 8: 590332, 2020.
  2. Limraksasin P, Kosaka Y, Zhang M, Horie N, Kondo T, Okawa H, Yamada M, Egusa H: Shaking culture enhances chondrogenic differentiation of mouse induced pluripotent stem cell constructs. Sci Rep, 10(1): 14996, 2020.
  3. Zhang M, Shi J, Xie M, Wen J, Niibe K, Zhang X, Luo J, Yan R, Zhang Z, Egusa H, Jiang X: Recapitulation of cartilage/bone formation using iPSCs via biomimetic 3D rotary culture approach for developmental engineering. Biomaterials, 260:120334, 2020.
  4. Xiang C, Tenkumo T, Ogawa T, Kanda Y, Nakamura K, Shirato M, Sokolova V, Epple M, Kamano Y, Egusa H, Sasaki K. Gene transfection achieved by utilizing antibacterial calcium phosphate nanoparticles for enhanced regenerative therapy. Acta Biomater, 119: 375-389, 2021.
  5. Zhang M, Zhang X, Luo J, Yan R, Niibe K, Egusa H, Zhang Z, Xie M, Jiang X: Investigate the odontogenic differentiation and dentin-Pulp tissue regeneration potential of neural crest cells. Front Bioeng Biotechnol, 8: 475, 2020.
  6. Manokawinchoke J, Sumrejkanchanakij P, Boonprakong L, Pavasant P, Egusa H, Osathanon T: NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells. Sci Rep, 10(1): 13329, 2020.
  7. Miura S, Yamauchi S, Kasahara S, Katsuda Y, Fujisawa M, Egusa H: Clinical evaluation of monolithic zirconia crowns: A failure analysis of clinically obtained cases from a 3.5-year study. J Prosthodont Res, accepted.
  8. Harada A, Shishido S, Barkarmo S, Inagaki R, Kanno T, Örtengren U, Egusa H, Nakamura K: Mechanical and microstructural properties of ultra-translucent dental zirconia ceramic stabilized with 5 mol% yttria. J Mech Behav Biomed Mater, 111:103974, 2020.
  9. Fakhruddin KS, Egusa H, Ngo HC, Panduwawala C, Pesee S, Venkatachalam T, Samaranayake LP: Silver diamine fluoride (SDF) used in childhood caries management has potent antifungal activity against oral Candida species. BMC Microbiol, 20(1): 95, 2020.
  10. Hong G, Chang TY, Terry A, Chuenjitwongsa S, Park YS, Tsoi KH, Sun JX, Kusdhany S, Egusa H, Yamada S, Kwon JS, Seow LL, Garcia CG, Loke WM, Auychai P, Hsu ML: Guidelines for the innovation of dental education under the coronavirus disease 2019 pandemic situation. J Oral Sci, 63(1): 107-110, 2020.
  11. Manokawinchoke J, Pavasant P, Limjeerajarus CN, Limjeerajarus N, Osathanon T, Egusa H: Mechanical loading and the control of stem cell behavior. Arch Oral Biol, accepted.
  12. Fakhruddin KS, Egusa H, Ngo HC, Panduwawala C, Pesee S, Samaranayake LP: Clinical efficacy and the antimicrobial potential of silver formulations in arresting dental caries: a systematic review. BMC Oral Health, 20(1): 160, 2020.
  13. Limraksasin P, Kondo T, Zhang M, Okawa H, Osathanon T, Pavasant P, Egusa H: In vitro fabrication of hybrid bone/cartilage complex using mouse induced pluripotent stem cells. Int J Mol Sci, 21(2): 581, 2020.
  14. Limraksasin P, Okawa H, Zhang M, Kondo T, Osathanon T, Pavasant P, Egusa H: Size-optimized micro-space culture facilitates differentiation of mouse induced pluripotent stem cells into osteoid-rich bone constructs. Stem Cells Int, Article ID 7082679, 2020.
  15. Yamada M, Watanabe J, Ueno T, Ogawa T, Egusa H: Cytoprotective preconditioning of osteoblast-Like cells with N-acetyl-L-cysteine for bone regeneration in cell therapy. Int J Mol Sci, 20(20):5199, 2019.
  16. Yamauchi S, Wang X, Egusa H, Sun J: High performance dental adhesives containing an ether-based monomer. J Dent Res, 99(2):189-195, 2020.
  17. Manokawinchoke J, Pavasant P, Sawangmake C, Limjeerajarus N, Limjeerajarus CN, Egusa H, Osathanon T: Intermittent compressive force promotes osteogenic differentiation in human periodontal ligament cells by regulating the transforming growth factor-β pathway. Cell Death Dis, 10(10): 761, 2019.
  18. Manokawinchoke J, Pavasant P, Sawangmake C, Limjeerajarus N, Limjeerajarus CN, Egusa H, Osathanon T: RNA sequencing data of human periodontal ligament cells treated with continuous and intermittent compressive force. Data Brief, 26: 104553, 2019.
  19. Watahiki A, Shimizu K, Hoshikawa S, Chiba M, Kitamura H, Egusa H, Fukumoto S, Inuzuka H: Lipin-2 degradation elicits a proinflammatory gene signature in macrophages. Biochem Biophys Res Commun, 524(2): 477-483, 2020.
  20. Fakhruddin KS, Samaranayake LP, Egusa H, Ngo HC, Panduwawala C, Venkatachalam T, Kumarappan A, Pesee S: Candida biome of severe early childhood caries (S-ECC) and its cariogenic virulence traits. J Oral Microbiol, 12(1): 1724484, 2020.
  21. Okawa H, Egusa H, Nishimura I: Implications of the circadian clock in implant dentistry. Dent Mater J, 39(2): 173-180, 2020.
  22. Nantavisai S, Egusa H, Osathanon T, Sawangmake C: Mesenchymal stem cell-based bone tissue engineering for veterinary practice. Heliyon, 5:e02808, 2019.
  23. Yamauchi S, Miura S, Kasahara S, Sun J, Egusa H: A thick frame decreases the fracture toughness of veneering ceramics used for zirconia-based all-ceramic restorations. J Prosthodont Res, 63:184-192, 2019.
  24. Watanabe J, Yamada M, Niibe K, Zhang M, Kondo T, Ishibashi M, Egusa H: Preconditioning of bone marrow-derived mesenchymal stem cells with N-acetyl-L-cysteine enhances bone regeneration via reinforced resistance to oxidative stress. Biomaterials, 185: 25-38, 2018.
  25. Nakazawa K, Nakamura K, Harada A, Shirato M, Inagaki R, Ortengren U, Kanno T, Niwano Y, Egusa H. Surface properties of dental zirconia ceramics affected by ultrasonic scaling and low-temperature degradation. PLoS One, 13(9): e0203849, 2018.
  26. Miura S, Kasahara S, Yamauchi S, Katsuda Y, Harada A, Aida J, Egusa H: A possible risk of CAD/CAM-produced composite resin premolar crowns on a removable partial denture abutment tooth: A 3-year retrospective cohort study. J Prosthodont Res, 63:78-84, 2019.
  27. Ankyu S, Nakamura K, Harada A, Inagaki R, Kastuda Y, Kanno T, Niwano Y, Ortengren U, Egusa H: Influence of microscale expansion and contraction caused by thermal and mechanical fatigue on retentive strength of CAD/CAM-generated resin-based composite crowns. J Mech Behav Biomed Mater, 86: 89-97, 2018.
  28. Zhang M, Yu W, Niibe K, Zhang W, Egusa H, Jiang X: The effects of platelet-derived growth factor-BB on bone marrow stromal cell-mediated vascularized bone regeneration. Stem Cells Int, Article ID 3272098, 2018.
  29. Shishido S, Miyano R, Nakashima T, Matsuo H, Iwatsuki M, Nakamura K, Kanno T, Egusa H, Niwano Y. A novel pathway for the photooxidation of catechin in relation to its prooxidative activity. Sci Rep, 8(1) 12888.
  30. Tenkumo T, Vanegas Saenz JR, Nakamura K, Shimuzu Y, Sokolova V, Epple M, Kamano Y, Egusa H, Sugaya T, Sasaki K: Prolonged release of bone morphogenetic protein-2 in vivo by gene transfection with DNA-functionalized calcium phosphate nanoparticle-loaded collagen scaffolds. Mater Sci Eng C Mater Biol Appl, 92: 172-183, 2018.
  31. Tateshima S, Kaneko N, Yamada M, Duckwiler G, Vinuela F, Ogawa T: Increased affinity of endothelial cells to NiTi using ultraviolet irradiation: An in vitro study. J Biomed Mater Res A, 106(4): 1034-1038, 2018.
  32. Kamano Y, Watanabe J, Iida T, Kondo T, Okawa H, Yatani H, Saeki M, Egusa H: Binding of PICK1 PDZ domain with calcineurin B regulates osteoclast differentiation. Biochem Biophys Res Commun, 496(1):83-88, 2018.
  33. Miura S, Kasahara S, Yamauchi S, Egusa H: Effect of finish line design on stress distribution in bilayer and monolithic zirconia crowns: A three-dimensional finite element analysis study. Eur J Oral Sci, 126(2):159-165, 2018. Miura S, Kasahara S, Yamauchi S, Okuyama Y, Izumida A, Aida J, Egusa H.
  34. Clinical evaluation of zirconia-based all-ceramic single crowns: an up to 12-year retrospective cohort study. Clin Oral Investig, 22(2): 697-706, 2018.
  35. Nowwarote N, Chanjavanakul P, Kongdecha P, Clayhan P, Egusa H, Pavasant P, Osathanon T. Characterization of a bioactive Jagged1-coated polycaprolactone-based membrane for guided tissue regeneration. Arch Oral Biol, 88:24-33, 2018.
  36. Manokawinchoke J, Nattasit P, Thongngam T, Pavasant P, Tompkins KA, Egusa H, Osathanon T. RNA sequencing data of Notch ligand treated human dental pulp cells. Data Brief, 17:407-413, 2018.
  37. Kayaba A, Itoh-Nakadai A, Niibe K, Shirota M, Funayama R, Sugahara-Tobinai A, Wong YL, Inui M, Nakayama K, Takai T. Bone marrow PDGFRα+Sca-1+-enriched mesenchymal stem cells support survival and antibody production by plasma cells in vitro through IL-6. Int Immunol. 30 (6):241-253, 2018.
  38. Yamada M, Egusa H: Current bone substitutes for implant dentistry. J Prosthodont Res, 62(2): 152-161, 2018.

Laboratory Contacts

Division Manager:Dr. Masahiro Yamada