Rehabilitation DentistryMolecular and Regenerative Prosthodontics

Our major research focus is the development of next-generation biotechnology to 1) regenerate missing alveolar bone and teeth for functional and esthetic rehabilitation using cells and biomimetic materials and 2) introduce tailor-made diagnostics for prosthetic and implant treatments to prevent further tooth loss.



    Prof. (Chair) Hiroshi Egusa,DDS, PhD, FRCPath


    Associate Prof. Masahiro Yamada,DDS, PhD

  • Senior Assist. Prof. Kunimichi Niibe,DDS, PhD

Topics of Research

  • iPS cell-based oral tissue engineering
  • Biomimetic materials for bone regeneration/dental implant
  • Osteo-immunology in alveolar bone resorption
  • Bone organoid fabrication focusing on clock genes
  • Development of therapeutic protein drugs for periodontal tissue regeneration
  • Development of genome-based diagnostics for prosthetic/implant treatments
  • Basic and clinical research on CAD/CAM-generated dental restorations
  • Basic/Clinical research for dental metal allergy
  • Development of AI evaluation system for tooth preparation

Recent Publications

  1. Watanabe J, Iwamatsu-kobayashi Y, Kikuchi K, Kajita T, Morishima H, Yamauchi K, Yashiro Q, Nishimura H, Kanetaka H, Egusa H: Visualization of droplets and aerosols in simulated dental treatments to clarify the effectiveness of oral suction devices. J Prosthodont Res, 68(1):85-91, 2024
  2. Kurihara A, Nakamura K, Shishido S, Inagaki R, Harada A, Kanno T, Egusa H: Mechanism underlying ultraviolet-irradiation-induced discoloration of dental zirconia ceramics stabilized with 3 and 5 mol% yttria. Ceram Int, in press.
  3. Kakinuma H, Koyama S, Kondo T, Harata T, Egusa H: In vitro evaluation of shape-memory hydrogels for removable dental prostheses and optimization of phase-transition temperature for intraoral use. J Prosthet Dent, in press.
  4. Kondo T, Otake K, Kakinuma H, SatoY, Ambo S, Egusa H: Contributed equally Zinc- and fluoride-releasing bioactive glass as a novel bone substitute. J Dent Res, in press.
  5. Kondo T, Yamada M, Egusa H: Innate immune regulation in dental implant osseointegration. J Prosthodont Res, in press.
  6. Kondo T, Kakinuma H, Fujimura K, Ambo S, Otake K, Sato Y, Egusa H: Incomplete polymerization of dual-cured resin cement due to attenuated light through zirconia induces inflammatory responses. Int J Mol Sci, 24(12): 9861, 2023.
  7. Tiskratok W, Yamada M, Watanabe J, Pengyu Q, Kimura T, Egusa H: Mechanoregulation of osteoclastogenesis-inducing potentials of fibrosarcoma cell line by substrate stiffness. Int J Mol Sci, 24(10): 8959, 2023.
  8. Nattasit P, Niibe K, Yamada M, Ohori-Morita Y, Limraksasin P, Tiskratok W, Yamamoto M, Egusa H: Stiffness-Tunable Hydrogel-Sandwich Culture Modulates the YAP-Mediated Mechanoresponse in Induced-Pluripotent Stem Cell Embryoid Bodies and Augments Cardiomyocyte Differentiation. Macromol Biosci, 23(7): e2300021, 2023.
  9. Tiskratok W, Yamada M, Watanabe J, Kartikasari N, Kimura T, Egusa H: Substrate stiffness controls proinflammatory responses in human gingival fibroblasts. Sci Rep, 13(1): 1358, 2023.
  10. Iwamatsu-Kobayashi Y, Watanabe J, Kusama T, Endo H, Ikeda S, Tokuda K, Igarashi K, Egusa H: A 19-year study of dental needlestick and sharps injuries in Japan. Int Dent J, 73(1): 114-120, 2023.
  11. Akashi Y, Nagasaki A, Okawa H, Matsumoto T, Kondo T, Yatani H, Nishimura I, Egusa H: Cyclic pressure-induced cytokines from gingival fibroblasts stimulate osteoclast activity: Clinical implications for alveolar bone loss in denture wearers. J Prosthodont Res, 67(1): 77-86, 2023.
  12. Limraksasin P, Nattasit P, Manokawinchoke J, Tiskratok W, Vinaikosol N, Okawa H, Limjeerajarus CN, Limjeerajarus N, Pavasant P, Osathanon T, Egusa H: Application of shear stress for enhanced osteogenic differentiation of mouse induced pluripotent stem cells. Sci Rep, 12(1): 19021, 2022.
  13. He X, Yamada M, Watanabe J, Tiskratok W, Ishibashi M, Kitaura H, Mizoguchi I, Egusa H. Titanium nanotopography induces osteocyte lacunar-canalicular networks to strengthen osseointegration. Acta Biomater, 151(1): 613-627, 2022.
  14. Kartikasari N, Yamada M, Watanabe J, Tiskratok W, He X, Egusa H: Titania nanospikes activate macrophage phagocytosis by ligand-independent contact stimulation. Sci Rep, 12(1): 12250, 2022.
  15. Zhang M, Niibe K, Kondo T, Limraksasin P, Okawa H, Miao X, Kamano Y, Yamada M, Jiang X, Egusa H: Rapid and efficient generation of cartilage pellets from mouse induced pluripotent stem cells by transcriptional activation of BMP-4 with shaking culture. J Tissue Eng, 13: 20417314221114616, 2022.
  16. Yamada M, Kimura T, Nakamura N, Watanabe J, Kartikasari N, He X, Tiskratok W, Yoshioka H, Shinno H, Egusa H: Titanium nanosurface with a biomimetic physical microenvironment to induce endogenous regeneration of the periodontium. ACS Appl Mater Interfaces, 14(24): 27703-27719, 2022.
  17. Miao X, Niibe K, Fu Y, Zhang M, Nattasit P, Ohori-Morita Y, Nakamura T, Jiang X, Egusa H: Epiprofin transcriptional activation promotes ameloblast induction from mouse induced pluripotent stem cells via the BMP-Smad signaling axis. Front Bioeng Biotechnol, 10: 890882, 2022.
  18. Inomata M, Harada A, Kasahara S, Kusama T, Ozaki A, Katsuda Y, Egusa H: Potential complications of CAD/CAM-produced resin composite crowns on molars: A retrospective cohort study over four years. PLoS One, 17(4): e0266358, 2022.
  19. Akaishi T, Kushimoto S, Katori Y, Sugawara N, Egusa H, Igarashi K, Fujita M, Kure S, Takayama S, Abe M, Kikuchi A, Ohsawa M, Ishizawa K, Abe Y, Imai H, Inaba Y, Iwamatsu-Kobayashi Y, Nishioka T, Onodera K, Ishii T: Effectiveness of third vaccine dose for coronavirus disease 2019 during the Omicron variant pandemic: a prospective observational study in Japan. Sci Rep, 12(1): 13589, 2022.
  20. Egusa H: Leadership for the further growth of JPR. J Prosthodont Res, 66(3): vi-vii, 2022.
  21. Kartikasari N, Yamada M, Watanabe J, Tiskratok W, He X, Kamano Y, Egusa H. Titanium surface with nanospikes tunes macrophage polarization to produce inhibitory factors for osteoclastogenesis through nanotopographic cues. Acta Biomater, 137: 316-330, 2022.
  22. Ohori-Morita Y, Niibe K, Limraksasin P, Nattasit P, Miao X, Yamada M, Mabuchi Y, Matsuzaki Y, Egusa H: Novel Mesenchymal Stem Cell Spheroids with Enhanced Stem Cell Characteristics and Bone Regeneration Ability. Stem Cells Transl Med, 11(4): 434-449, 2022.
  23. Fakhruddin KS, Samaranayake LP, Hamoudi RA, Ngo HC, Egusa H: Diversity of site-specific microbes of occlusal and proximal lesions in severe- early childhood caries (S-ECC). J Oral Microbiol, 14(1): 2037832, 2022.
  24. Fukuyasu S, Kayashima H, Moribayashi A, Matsuoka S, Nagasaki A, Okawa H, Yatani H, Saeki M, Egusa H. Cell-based double-screening method to identify a reliable candidate for osteogenesis-targeting compounds. Biomedicines, 10:426, 2022.
  25. Egusa H: JPR starts 2022 with a new design -Towards a new frontier in prosthodontics. J Prosthodont Res, 66(1): vi-vii, 2022.
  26. Limraksasin P, Egusa H: A method for in vitro fabrication of hybrid bone/cartilage tissue using mouse induced pluripotent stem cells. iPS Cells: Methods and Protocols 2nd Edition. Springer Nature, 2454: 509-520, 2022.
  27. Miao X, Niibe K, Zhang M, Liu Z, Nattasit P, Ohori-Morita Y, Nakamura T, Jiang X, Egusa H: Stage-specific role of amelx activation in stepwise ameloblast induction from mouse induced pluripotent stem cells. Int J Mol Sci, 22(13): 7195, 2021.
  28. Ozaki A, Shishido S, Nakamura K, Harada A, Katsuda Y, Kanno T, Egusa H: Impact of adhesive primer and light-curing on polymerization kinetics of self-adhesive resin cement in association with free radical reaction. Eur J Oral Sci, 129(6): e12828, 2021.
  29. Egusa H: JPR continues to grow as a leading dental journal and international public asset. J Prosthodont Res, 65(3): vi, 2021.
  30. Osathanon T, Egusa H: Notch signaling in induced pluripotent stem cells. Molecular Players in iPSC Technology, Volume 12, 1st Edition. Ed: Birbrair A, Elsevier, Amsterdam, 249-284, 2021.
  31. Miura S, Yamauchi S, Kasahara S, Katsuda Y, Fujisawa M, Egusa H: Clinical evaluation of monolithic zirconia crowns: A failure analysis of clinically obtained cases from a 3.5-year study. J Prosthodont Res, 65(2): 148-154, 2021.
  32. Manokawinchoke J, Pavasant P, Limjeerajarus CN, Limjeerajarus N, Osathanon T, Egusa H: Mechanical loading and the control of stem cell behavior. Arch Oral Biol,125: 105092, 2021.
  33. Niibe K, Ohori-Morita Y, Zhang M, Mabuchi Y, Matsuzaki Y, Egusa H: A shaking-culture method for generating bone marrow derived mesenchymal stromal/stem cell-spheroids with enhanced multipotency in vitro. Front Bioeng Biotechnol, 8: 590332, 2020.
  34. Limraksasin P, Kosaka Y, Zhang M, Horie N, Kondo T, Okawa H, Yamada M, Egusa H: Shaking culture enhances chondrogenic differentiation of mouse induced pluripotent stem cell constructs. Sci Rep, 10(1): 14996, 2020.
  35. Harada A, Shishido S, Barkarmo S, Inagaki R, Kanno T, Örtengren U, Egusa H, Nakamura K: Mechanical and microstructural properties of ultra-translucent dental zirconia ceramic stabilized with 5 mol% yttria. J Mech Behav Biomed Mater, 111: 103974, 2020.
  36. Hong G, Chang TY, Terry A, Chuenjitwongsa S, Park YS, Tsoi KH, Sun JX, Kusdhany S, Egusa H, Yamada S, Kwon JS, Seow LL, Garcia CG, Loke WM, Auychai P, Hsu ML: Guidelines for the innovation of dental education under the coronavirus disease 2019 pandemic situation. J Oral Sci, 63(1): 107-110, 2020.
  37. Limraksasin P, Kondo T, Zhang M, Okawa H, Osathanon T, Pavasant P, Egusa H: In vitro fabrication of hybrid bone/cartilage complex using mouse induced pluripotent stem cells. Int J Mol Sci, 21(2): 581, 2020.
  38. Limraksasin P, Okawa H, Zhang M, Kondo T, Osathanon T, Pavasant P, Egusa H: Size-optimized micro-space culture facilitates differentiation of mouse induced pluripotent stem cells into osteoid-rich bone constructs. Stem Cells Int, Article ID 7082679, 2020.
  39. Watahiki A, Shimizu K, Hoshikawa S, Chiba M, Kitamura H, Egusa H, Fukumoto S, Inuzuka H: Lipin-2 degradation elicits a proinflammatory gene signature in macrophages. Biochem Biophys Res Commun, 524(2): 477-483, 2020.
  40. Okawa H, Egusa H, Nishimura I: Implications of the circadian clock in implant dentistry. Dent Mater J, 39(2): 173-180, 2020.
  41. Yamada M, Watanabe J, Ueno T, Ogawa T, Egusa H: Cytoprotective preconditioning of osteoblast-Like cells with N-acetyl-L-cysteine for bone regeneration in cell therapy. Int J Mol Sci, 20(20): 5199, 2019.
  42. Yamauchi S, Miura S, Kasahara S, Sun J, Egusa H: A thick frame decreases the fracture toughness of veneering ceramics used for zirconia-based all-ceramic restorations. J Prosthodont Res, 63: 184-192, 2019.
  43. Miura S, Kasahara S, Yamauchi S, Katsuda Y, Harada A, Aida J, Egusa H: A possible risk of CAD/CAM-produced composite resin premolar crowns on a removable partial denture abutment tooth: A 3-year retrospective cohort study. J Prosthodont Res, 63: 78-84, 2019.
  44. Watanabe J, Yamada M, Niibe K, Zhang M, Kondo T, Ishibashi M, Egusa H: Preconditioning of bone marrow-derived mesenchymal stem cells with N-acetyl-L-cysteine enhances bone regeneration via reinforced resistance to oxidative stress. Biomaterials, 185: 25-38, 2018.
  45. Nakazawa K, Nakamura K, Harada A, Shirato M, Inagaki R, Ortengren U, Kanno T, Niwano Y, Egusa H: Surface properties of dental zirconia ceramics affected by ultrasonic scaling and low-temperature degradation. PLoS One, 13(9): e0203849, 2018.
  46. Ankyu S, Nakamura K, Harada A, Inagaki R, Kastuda Y, Kanno T, Niwano Y, Ortengren U, Egusa H: Influence of microscale expansion and contraction caused by thermal and mechanical fatigue on retentive strength of CAD/CAM-generated resin-based composite crowns. J Mech Behav Biomed Mater, 86: 89-97, 2018.
  47. Zhang M, Yu W, Niibe K, Zhang W, Egusa H, Jiang X: The effects of platelet-derived growth factor-BB on bone marrow stromal cell-mediated vascularized bone regeneration. Stem Cells Int, Article ID 3272098, 2018.
  48. Shishido S, Miyano R, Nakashima T, Matsuo H, Iwatsuki M, Nakamura K, Kanno T, Egusa H, Niwano Y: A novel pathway for the photooxidation of catechin in relation to its prooxidative activity. Sci Rep, 8(1): 12888, 2018.
  49. Tenkumo T, Vanegas Saenz JR, Nakamura K, Shimuzu Y, Sokolova V, Epple M, Kamano Y, Egusa H, Sugaya T, Sasaki K: Prolonged release of bone morphogenetic protein-2 in vivo by gene transfection with DNA-functionalized calcium phosphate nanoparticle-loaded collagen scaffolds. Mater Sci Eng C Mater Biol Appl, 92: 172-183, 2018.
  50. Tateshima S, Kaneko N, Yamada M, Duckwiler G, Vinuela F, Ogawa T: Increased affinity of endothelial cells to NiTi using ultraviolet irradiation: An in vitro study. J Biomed Mater Res A, 106(4): 1034-1038, 2018.

Laboratory Contacts

Division Manager|Dr. Masahiro Yamada