Rehabilitation DentistryCraniofacial Development and Tissue Biology

Listed below are the themes of research we (in Craniofacial Development and Tissue Biology) perform. Our research is closely related to dentistry and focuses on bones and teeth. We also do our best to raise our students to be highly specialized professionals in dentistry by holding lectures on the human body, oral cavity and structure of teeth tissue.

(1) Research on the development, repair and regeneration of bones and teeth. A modified version of basic biological phenomena seen in an early development stage is used during the process of tissue repair and regeneration. We aim to identify the shared and unique traits of the two processes.

(2) Bones and teeth have abundant extracellular matrices. Extracellular matrix controls the basis of tissue differentiation and determines the speciality of differentiated tissues. Under the assumption they impact tissue differentiation, we research what functions the various molecules in extracellular matrix have, and when, where and how they work.

(3) We research the phenomenon of the hardening of teeth and bones - calcification. Calcification occurs when the crystals of calcium phosphate deposit on extracellular matrix. We seek to identify the roles of cells and extracellular matrix in the process of calcification.


Topics of Research

  • Development, regeneration and healing of bones and teeth
  • Regulatory mechanisms of calcification
  • Remodeling of extracellular matrices in calcified tissues
  • Regulatory mechanisms of extracellular matrices on cell differentiation in calcified tissues

Recent Publications

  1. Nakamura M, Aoyama N, Yamaguchi S, Sasano Y (2021) Expression of tartrate-resistant acid phosphatase and cathepsin K during osteoclast differentiation in developing mouse mandibles. Biomedical Research-Tokyo 42(1) 13-21.
  2. Sasano Y, Nakamura M, Henmi A, Okata H, Suzuki O, Kayaba A, Mayanagi M (2019) Degradation of extracellular matrices propagates calcification during development and healing in bones and teeth. Journal of Oral Biosciences 61:149-156.
  3. Otake Y, Nakamura M, Henmi A, Takahashi T, Sasano Y (2018) Experimental Comparison of the Performance of Cutting Bone and Soft Tissue between Piezosurgery and Conventional Rotary Instruments. Scientific ReportsNov 21;8(1):17154.
  4. Henmi A, Okata H, Mikami Y, Sasano Y (2017) Calcification in rat developing mandibular bone demonstrated by whole mount staining, micro-computed tomography and scanning electron microscopy with energy dispersive X-ray spectroscopy. Biomedical Research-Tokyo 38(5): 277–284.
  5. Henmi A, Okata H, Anada T, Yoshinari M, Mikami Y, Suzuki O, Sasano Y (2016) Bone matrix calcification during embryonic and postembryonic rat calvarial development assessed by SEM-EDX, XRD and FTIR. J Bone Miner Metab 34:41–50.
  6. Okata H, Nakamura M, Henmi A, Yamaguchi S, Mikami Y, Shimauchi H, Sasano Y (2015) Calcification during bone healing in a standardised rat calvarial defect assessed by micro-CT and SEM-EDX. Oral Diseases 21: 74-82.
  7. Henmi A, Nakamura M, Echigo S, Sasano Y (2011) Involvement of sensory neuron in bone defect repair in rats. J Electron Microsc 60:393-400.
  8. Kimura Y, Kikunaga S, Takahashi I, Hatakeyama Y, Fukumoto S, Sasano Y (2011) Characterization of the calcification process modeled in rat embryonic calvarial culture. J Electron Microsc 60:345-52.
  9. Andou K, Nakamura M, Ina Y, Sasaki K, Sasano Y (2011) Root and periodontal tissue development after allogenic tooth transplantation between rat littermates. Oral Diseases 17:379-386.
  10. Ebina H, Hatakeyama J, Onodera M, Honma T, Kamakura S, Shimauchi H, Sasano Y (2009) Micro-CT analysis of alveolar bone healing using a rat experimental model of critical-size defects. Oral Diseases 15:273–280.
  11. Itagaki T, Honma T, Takahashi I, Echigo S, Sasano Y (2008) Quantitative analysis and localization of mRNA transcripts of type I collagen, osteocalcin, MMP 2, MMP 8 and MMP 13 during bone healing in a rat calvarial experimental defect model. Anatomical Record—Advances in Integrative Anatomy and Evolutionary Biology 291:1038-1046.
  12. Honma T, Itagaki T, Nakamura M, Kamakura S, Tkahashi I, Echigo S, Sasano Y (2008) Bone formation in rat calvaria ceases within a limited period regardless of completion of defect repair. Oral Diseases 14: 457-464.

Laboratory Contacts