Joint Research DepartmentAdvanced Free Radical Science

Department of Advanced Free Radical Science is a joint research department established by Luke co. ltd. The purpose of our research is to develop novel medical and dental devices utilizing antimicrobial effect of free radicals and/or reactive oxygen species. In particular, bactericidal activity of hydroxyl radicals generated by photolysis of hydrogen peroxide has been studied, and we have developed a therapeutic device for periodontal treatment based on this technique. The efficacy and safety of this device has been demonstrated via an investigator-initiated clinical trial (Kanno et al. Sci Rep, 7, 12247, 2017). Currently, we are filing the application for product approval in collaboration with the company.


Topics of Research

  • R&D of dental therapeutic devices based on antimicrobial technique using free radicals.
  • Development of antimicrobial techniques utilizing pro-oxidative activity of polyphenols.
  • Evaluation of mechanical and biological properties of dental materials.

Recent Publications

  1. Kurihara A, Nakamura K, Shishido S, Inagaki R, Harada A, Kanno T, Egusa H. Mechanism underlying ultraviolet-irradiation-induced discoloration of dental zirconia ceramics stabilized with 3 and 5 mol% yttria. Ceram Int. Online ahead of print, 2024
  2. Shishido S, Inagaki R, Kanno T, Svanborg P, Barkarmo S, Örtengren U, Nakamura K. Residual stress associated with crystalline phase transformation of 3–6 mol% yttria-stabilized zirconia ceramics induced by mechanical surface treatments. J Mech Behav Biomater Med. 146:106067, 2023
  3. Shirato M, Takida Y, Kanno T, Matsuura H, Niwano Y, Minamide H, Nakamura K. Mutagenicity assessment of high-power 1.6 THz pulse laser radiation. Photochem Photobiol. 100: 146-158, 2024
  4. Niwano Y, Kohzaki H, Shirato M, Shishido S, Nakamura K. Metabolic fate of orally ingested proanthocyanidins through the digestive tract. Antioxidants, 12: 17, 2023
  5. Shirato M, Nakamura K, Tenkumo T, Niwano Y, Kanno T, Sasaki K, Lingström P, Örtengren U. Inhibition of tooth demineralization caused by Streptococcus mutans biofilm via antimicrobial treatment using hydrogen peroxide photolysis. Clin Oral Invest, 27: 739-750, 2023
  6. Harai T, Mizutani M, Shishido S, Nakamura K, Ohmori H, Konno T, Kuriyagawa T. Low-temperature degradation of yttria-stabilized zirconia treated with pulsed laser and annealing techniques. Precis Eng, 80: 45-56, 2023
  7. Niwano Y, Kohzaki H, Shirato M, Shishido S, Nakamura K. Putative mechanisms underlying beneficial effects of polyphenols in murine models of metabolic disorders in relation to gut microbiota. Curr Issues Mol Biol, 44: 1353-1375, 2022
  8. Niwano Y, Kohzaki H, Shirato M, Shishido S, Nakamura K. Anti-osteoporotic mechanisms of polyphenols elucidated based on in vivo studies using ovariectomized animals. Antioxidants, 11: 217, 2022
  9. Nakamura K, Tarkeshi A, Niklasson A, Shishido S, Svanborg P, Barkarmo S, Örtengren U. Influence of crystalline phase transformation induced by airborne-particle abrasion and low-temperature degradation on mechanical properties of dental zirconia ceramics stabilized with over 5 mol% yttria. J Mech Behave Biomed Mater, 125, 104890, 2021
  10. Nakamura K, Shirato M, Shishido S, Kanno T, Niwano Y, Sasaki K, Lingström P, Örtengren U. Reactions of dental pulp to hydrogen peroxide photolysis-based antimicrobial chemotherapy under ultraviolet-A irradiation in rats. J Photochem Photobiol B, 212: 112042, 2020

Laboratory Contacts