Joint Research DepartmentAdvanced Free Radical Science

Department of Advanced Free Radical Science is a joint research department established by Luke co. ltd. The purpose of our research is to develop novel medical and dental devices utilizing antimicrobial effect of free radicals and/or reactive oxygen species. In particular, bactericidal activity of hydroxyl radicals generated by photolysis of hydrogen peroxide has been studied, and we have developed a therapeutic device for periodontal treatment based on this technique. The efficacy and safety of this device has been demonstrated via an investigator-initiated clinical trial (Kanno et al. Sci Rep, 7, 12247, 2017). Currently, we are filing the application for product approval in collaboration with the company.


Topics of Research

  • R&D of dental therapeutic devices based on antimicrobial technique using free radicals.
  • Development of antimicrobial techniques utilizing pro-oxidative activity of polyphenols.
  • Evaluation of mechanical and biological properties of dental materials.

Recent Publications

  1. Niwano Y, Kohzaki H, Shirato M, Shishido S, Nakamura K. Anti-osteoporotic mechanisms of polyphenols elucidated based on in vivo studies using ovariectomized animals. Antioxidants, 11: 217, 2022 
  2. Nakamura K, Tarkeshi A, Niklasson A, Shishido S, Svanborg P, Barkarmo S, Örtengren U. Influence of crystalline phase transformation induced by airborne-particle abrasion and low-temperature degradation on mechanical properties of dental zirconia ceramics stabilized with over 5 mol% yttria. J Mech Behave Biomed Mater, 125, 104890, 2021
  3. Nakamura K, Shirato M, Shishido S, Kanno T, Niwano Y, Sasaki K, Lingström P, Örtengren U. Reactions of dental pulp to hydrogen peroxide photolysis-based antimicrobial chemotherapy under ultraviolet-A irradiation in rats. J Photochem Photobiol B, 212: 112042, 2020
  4. Shirato M, Nakamura K, Tenkumo T, Kano Y, Ishiyama K, Kanno T, Sasaki K, Niwano Y, Matsuura H. Oral mucosal irritation potential of antimicrobial chemotherapy involving hydrogen peroxide photolysis with high-power laser irradiation for the treatment of periodontitis. J Photochem Photobiol B, 201: 111633, 2019
  5. Nakamura K, Shirato M, Tenkumo T, Kanno T, Westerlund A, Örtengren U, Sasaki K, Niwano Y. Hydroxyl radicals generated by hydrogen peroxide photolysis recondition biofilm-contaminated titanium surfaces for subsequent osteoblastic cell proliferation. Sci Rep, 9: 4688, 2019
  6. Shishido S, Miyano R, Nakashima T, Matsuo H, Iwatsuki M, Nakamura K, Kanno T, Egusa H, Niwano Y. A novel pathway for the photooxidation of catechin in relation to its prooxidative activity. Sci Rep, 8: 12888, 2018
  7. Kanno T, Nakamura K, Ishiyama K, Yamada Y, Shirato M, Niwano Y, Kayaba C, Ikeda K, Takagi A, Yamaguchi T, Sasaki K. Adjunctive antimicrobial chemotherapy based on hydrogen peroxide photolysis in non-surgical treatment for moderate to severe periodontitis: a randomized clinical trial. Sci Rep, 7: 12247, 2017

Laboratory Contacts